cd-1 mouse cortical neurons m-cx-400 (Lonza)
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Cd 1 Mouse Cortical Neurons M Cx 400, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Article Title: Transcriptional expression patterns triggered by chemically distinct neuroprotective molecules
Journal: Neuroscience
doi: 10.1016/j.neuroscience.2012.09.007
Figure Legend Snippet: IRF7 Expression Following Excitotoxicity and RNAi. Irf7 and Sema3a expression in neuronal samples either before NMDA excitotoxic stimulus (Pre) or after stimulus and a 16-hr recovery period (Post). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. qRT-PCR ΔΔCt quantitation method: calibrator, GAPDH; reference, pre-assay mock.
Techniques Used: Expressing, Transfection, Negative Control, Quantitative RT-PCR, Quantitation Assay
Figure Legend Snippet: Survival Effects of IRF7 RNAi and Type-I Interferons. A. Neuronal surival following Irf7 RNAi. Survival assay following 16hr recovery period for transfected neurons. N = 3. * p < 10−6 (Student’s t-test). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. B. Survival assay (see materials in method) in presence of type-I IFNs (left to right) NT = vehicle control, IFNA = IFN-α, IFNB = IFN-β (N=4), MK-801, positive control NMDA receptor anatagonist.
Techniques Used: Clonogenic Cell Survival Assay, Transfection, Negative Control, Control, Positive Control
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