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cd-1 mouse cortical neurons m-cx-400  (Lonza)


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    Structured Review

    Lonza cd-1 mouse cortical neurons m-cx-400
    <t>IRF7</t> Expression Following Excitotoxicity and RNAi. Irf7 and Sema3a expression in neuronal samples either before NMDA excitotoxic stimulus (Pre) or after stimulus and a 16-hr recovery period (Post). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. qRT-PCR ΔΔCt quantitation method: calibrator, GAPDH; reference, pre-assay mock.
    Cd 1 Mouse Cortical Neurons M Cx 400, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd-1+mouse+cortical+neurons/cortical++m+cx+400++primary+neuronal+cells/pmc03489981-199-4-10
    Average 90 stars, based on 1 article reviews
    cd-1 mouse cortical neurons m-cx-400 - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Transcriptional expression patterns triggered by chemically distinct neuroprotective molecules"

    Article Title: Transcriptional expression patterns triggered by chemically distinct neuroprotective molecules

    Journal: Neuroscience

    doi: 10.1016/j.neuroscience.2012.09.007

    IRF7 Expression Following Excitotoxicity and RNAi. Irf7 and Sema3a expression in neuronal samples either before NMDA excitotoxic stimulus (Pre) or after stimulus and a 16-hr recovery period (Post). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. qRT-PCR ΔΔCt quantitation method: calibrator, GAPDH; reference, pre-assay mock.
    Figure Legend Snippet: IRF7 Expression Following Excitotoxicity and RNAi. Irf7 and Sema3a expression in neuronal samples either before NMDA excitotoxic stimulus (Pre) or after stimulus and a 16-hr recovery period (Post). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. qRT-PCR ΔΔCt quantitation method: calibrator, GAPDH; reference, pre-assay mock.

    Techniques Used: Expressing, Transfection, Negative Control, Quantitative RT-PCR, Quantitation Assay

    Survival Effects of IRF7 RNAi and Type-I Interferons. A. Neuronal surival following Irf7 RNAi. Survival assay following 16hr recovery period for transfected neurons. N = 3. * p < 10−6 (Student’s t-test). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. B. Survival assay (see materials in method) in presence of type-I IFNs (left to right) NT = vehicle control, IFNA = IFN-α, IFNB = IFN-β (N=4), MK-801, positive control NMDA receptor anatagonist.
    Figure Legend Snippet: Survival Effects of IRF7 RNAi and Type-I Interferons. A. Neuronal surival following Irf7 RNAi. Survival assay following 16hr recovery period for transfected neurons. N = 3. * p < 10−6 (Student’s t-test). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. B. Survival assay (see materials in method) in presence of type-I IFNs (left to right) NT = vehicle control, IFNA = IFN-α, IFNB = IFN-β (N=4), MK-801, positive control NMDA receptor anatagonist.

    Techniques Used: Clonogenic Cell Survival Assay, Transfection, Negative Control, Control, Positive Control

    Related Articles

    Transfection:

    Article Title: Transcriptional expression patterns triggered by chemically distinct neuroprotective molecules
    Article Snippet: Assays were performed in triplicate using ~50 ng of RNA per reaction and reported using the ΔΔC t comparative method as detailed in the ABI User Bulletin #2, using Gapdh as the sample calibrator and the references (relative controls) indicated in respective figures. .. Neuronal Transfection and RNA Interference For functional assays following Irf7 interference, CD-1 mouse cortical neurons (Lonza, M-Cx-400) were cultured according to the manufacturer’s protocol. ..

    Functional Assay:

    Article Title: Transcriptional expression patterns triggered by chemically distinct neuroprotective molecules
    Article Snippet: Assays were performed in triplicate using ~50 ng of RNA per reaction and reported using the ΔΔC t comparative method as detailed in the ABI User Bulletin #2, using Gapdh as the sample calibrator and the references (relative controls) indicated in respective figures. .. Neuronal Transfection and RNA Interference For functional assays following Irf7 interference, CD-1 mouse cortical neurons (Lonza, M-Cx-400) were cultured according to the manufacturer’s protocol. ..

    Cell Culture:

    Article Title: Transcriptional expression patterns triggered by chemically distinct neuroprotective molecules
    Article Snippet: Assays were performed in triplicate using ~50 ng of RNA per reaction and reported using the ΔΔC t comparative method as detailed in the ABI User Bulletin #2, using Gapdh as the sample calibrator and the references (relative controls) indicated in respective figures. .. Neuronal Transfection and RNA Interference For functional assays following Irf7 interference, CD-1 mouse cortical neurons (Lonza, M-Cx-400) were cultured according to the manufacturer’s protocol. ..



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    Lonza cd-1 mouse cortical neurons m-cx-400
    <t>IRF7</t> Expression Following Excitotoxicity and RNAi. Irf7 and Sema3a expression in neuronal samples either before NMDA excitotoxic stimulus (Pre) or after stimulus and a 16-hr recovery period (Post). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. qRT-PCR ΔΔCt quantitation method: calibrator, GAPDH; reference, pre-assay mock.
    Cd 1 Mouse Cortical Neurons M Cx 400, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd-1+mouse+cortical+neurons/cortical++m+cx+400++primary+neuronal+cells/pmc03489981-199-4-10
    Average 90 stars, based on 1 article reviews
    cd-1 mouse cortical neurons m-cx-400 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

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    Lonza cd-1 mouse cortical neurons
    <t>IRF7</t> Expression Following Excitotoxicity and RNAi. Irf7 and Sema3a expression in neuronal samples either before NMDA excitotoxic stimulus (Pre) or after stimulus and a 16-hr recovery period (Post). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. qRT-PCR ΔΔCt quantitation method: calibrator, GAPDH; reference, pre-assay mock.
    Cd 1 Mouse Cortical Neurons, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd-1+mouse+cortical+neurons/cd+1+mouse+cortical+neurons/pmc03489981-155-9-15
    Average 90 stars, based on 1 article reviews
    cd-1 mouse cortical neurons - by Bioz Stars, 2026-09
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      Buy from Supplier

    Image Search Results


    IRF7 Expression Following Excitotoxicity and RNAi. Irf7 and Sema3a expression in neuronal samples either before NMDA excitotoxic stimulus (Pre) or after stimulus and a 16-hr recovery period (Post). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. qRT-PCR ΔΔCt quantitation method: calibrator, GAPDH; reference, pre-assay mock.

    Journal: Neuroscience

    Article Title: Transcriptional expression patterns triggered by chemically distinct neuroprotective molecules

    doi: 10.1016/j.neuroscience.2012.09.007

    Figure Lengend Snippet: IRF7 Expression Following Excitotoxicity and RNAi. Irf7 and Sema3a expression in neuronal samples either before NMDA excitotoxic stimulus (Pre) or after stimulus and a 16-hr recovery period (Post). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. qRT-PCR ΔΔCt quantitation method: calibrator, GAPDH; reference, pre-assay mock.

    Article Snippet: For functional assays following Irf7 interference, CD-1 mouse cortical neurons (Lonza, M-Cx-400) were cultured according to the manufacturer’s protocol.

    Techniques: Expressing, Transfection, Negative Control, Quantitative RT-PCR, Quantitation Assay

    Survival Effects of IRF7 RNAi and Type-I Interferons. A. Neuronal surival following Irf7 RNAi. Survival assay following 16hr recovery period for transfected neurons. N = 3. * p < 10−6 (Student’s t-test). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. B. Survival assay (see materials in method) in presence of type-I IFNs (left to right) NT = vehicle control, IFNA = IFN-α, IFNB = IFN-β (N=4), MK-801, positive control NMDA receptor anatagonist.

    Journal: Neuroscience

    Article Title: Transcriptional expression patterns triggered by chemically distinct neuroprotective molecules

    doi: 10.1016/j.neuroscience.2012.09.007

    Figure Lengend Snippet: Survival Effects of IRF7 RNAi and Type-I Interferons. A. Neuronal surival following Irf7 RNAi. Survival assay following 16hr recovery period for transfected neurons. N = 3. * p < 10−6 (Student’s t-test). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. B. Survival assay (see materials in method) in presence of type-I IFNs (left to right) NT = vehicle control, IFNA = IFN-α, IFNB = IFN-β (N=4), MK-801, positive control NMDA receptor anatagonist.

    Article Snippet: For functional assays following Irf7 interference, CD-1 mouse cortical neurons (Lonza, M-Cx-400) were cultured according to the manufacturer’s protocol.

    Techniques: Clonogenic Cell Survival Assay, Transfection, Negative Control, Control, Positive Control

    IRF7 Expression Following Excitotoxicity and RNAi. Irf7 and Sema3a expression in neuronal samples either before NMDA excitotoxic stimulus (Pre) or after stimulus and a 16-hr recovery period (Post). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. qRT-PCR ΔΔCt quantitation method: calibrator, GAPDH; reference, pre-assay mock.

    Journal: Neuroscience

    Article Title: Transcriptional expression patterns triggered by chemically distinct neuroprotective molecules

    doi: 10.1016/j.neuroscience.2012.09.007

    Figure Lengend Snippet: IRF7 Expression Following Excitotoxicity and RNAi. Irf7 and Sema3a expression in neuronal samples either before NMDA excitotoxic stimulus (Pre) or after stimulus and a 16-hr recovery period (Post). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. qRT-PCR ΔΔCt quantitation method: calibrator, GAPDH; reference, pre-assay mock.

    Article Snippet: Neuronal Transfection and RNA Interference For functional assays following Irf7 interference, CD-1 mouse cortical neurons (Lonza, M-Cx-400) were cultured according to the manufacturer’s protocol.

    Techniques: Expressing, Transfection, Negative Control, Quantitative RT-PCR, Quantitation Assay

    Survival Effects of IRF7 RNAi and Type-I Interferons. A. Neuronal surival following Irf7 RNAi. Survival assay following 16hr recovery period for transfected neurons. N = 3. * p < 10−6 (Student’s t-test). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. B. Survival assay (see materials in method) in presence of type-I IFNs (left to right) NT = vehicle control, IFNA = IFN-α, IFNB = IFN-β (N=4), MK-801, positive control NMDA receptor anatagonist.

    Journal: Neuroscience

    Article Title: Transcriptional expression patterns triggered by chemically distinct neuroprotective molecules

    doi: 10.1016/j.neuroscience.2012.09.007

    Figure Lengend Snippet: Survival Effects of IRF7 RNAi and Type-I Interferons. A. Neuronal surival following Irf7 RNAi. Survival assay following 16hr recovery period for transfected neurons. N = 3. * p < 10−6 (Student’s t-test). Neurons were transfected 24-hr prior to excitotoxic stimulus with either Mock (vehicle), negative control siRNA (NegCon), or Irf7 specific siRNA oligo. B. Survival assay (see materials in method) in presence of type-I IFNs (left to right) NT = vehicle control, IFNA = IFN-α, IFNB = IFN-β (N=4), MK-801, positive control NMDA receptor anatagonist.

    Article Snippet: Neuronal Transfection and RNA Interference For functional assays following Irf7 interference, CD-1 mouse cortical neurons (Lonza, M-Cx-400) were cultured according to the manufacturer’s protocol.

    Techniques: Clonogenic Cell Survival Assay, Transfection, Negative Control, Control, Positive Control